Bulletin of Experimental Biology and Medicine

, Volume 81, Issue 3, pp 390–392 | Cite as

Some properties of low-molecular-weight tetanus antitoxin

  • Ya. I. Aleksevich
  • G. N. Kryzhanovskii
Microbiology and Immunology


After intravenous injection of tetanus antitoxin obtained by tryptic digestion of “Diaferm-3” horse immunoglobulin, purification, and concentration of the active fragments, the antitoxin was excreted by rabbits 3 times faster than after injection of the original “Diaferm-3” antitoxin. After injection of the fragmented antitoxin its excretion continued until the 6th days, whereas after injection of “Diaferm-3” antitoxin its excretion continued until the 19th day; in the first case much less antitoxin was excreted than in the second (2% and 3.5% respectively). In both cases the antitoxin excreted in the urine consisted of monovalent Fab' fragments which caused delay of precipitation in the cross reaction in agar gel between tetanus toxoid and antitetanus serum. The Fab' gragment obtained by this method possessed anaphylactogenic properties.

Key Words

low-molecular-weight antibodies tetanus tetanus antitoxin 


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Literature Cited

  1. 1.
    G. N. Kryzhanovskii and L. P. Alekseev, Zh. Mikrobiol., No. 1, 74 (1969).Google Scholar
  2. 2.
    G. N. Kryzhanovskii, L. P. Alekseev, A. Ya. Kul'berg, et al., Byull. Éksperim. Biol. i Med., No. 7, 77 (1967).Google Scholar
  3. 3.
    G. N. Kryzhanovskii, L. N. Fontalin, and L. A. Pevnitskii, Vestn. Akad. Med. Nauk SSSR, No. 10, 18 (1960).Google Scholar
  4. 4.
    A. Ya. Kul'berg, Uspekhi Sovr. Biol.,76, No. 1, 110 (1973).Google Scholar
  5. 5.
    A. Ya. Kul'berg and L. M. Bartova, Vopr. Med. Khimii, No. 5, 514 (1963).Google Scholar
  6. 6.
    E. Ya. Severova and T. D. Margulis, Ter. Arkh., No. 11, 109 (1971).Google Scholar
  7. 7.
    L. A. Chernaya and S. V. Yaremko, Vopr. Immunol., No. 3, 52 (1968).Google Scholar
  8. 8.
    F. A. Chertkova and T. V. Shapovalova, in: Proceedings of an Inter-Institute Scientific Conference in Memory of L. A. Tarasevich [in Russian], Moscow (1967), pp. 29–30.Google Scholar
  9. 9.
    F. J. Dixon, D. W. Talmage, P. H. Maurer, et al., J. Exp. Med.,96, 313 (1952).Google Scholar
  10. 10.
    E. Falchetti, C. R. Soc. Biol.,124, 96 (1937).Google Scholar
  11. 11.
    R. H. Regamey, Schweiz. Z. allg. Path.,16, 873 (1953).Google Scholar
  12. 12.
    J. S. Remington, E. Merler, A. M. Lerner, et al., Nature,194, 407 (1962).Google Scholar
  13. 13.
    H. E. Schultze, H. Haupt, E. Heide, et al., Immunochemistry,2, 273 (1965).Google Scholar
  14. 14.
    H. L. Spiegelberg and W. O. Weigle, Immunochemistry,3, 341 (1966).Google Scholar
  15. 15.
    W. O. Weigle, in: Mechanisms of Antibody Formation, Prague (1960), p. 53.Google Scholar

Copyright information

© Plenum Publishing Corporation 1976

Authors and Affiliations

  • Ya. I. Aleksevich
  • G. N. Kryzhanovskii
    • 1
  1. 1.Academy of Medical Sciences of the USSRUSSR

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