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Isolation and immunochemical analysis of hydrolysis products of γG-globulin in the serum of partially hepatectomized rabbits

  • Microbiology and Immunology
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

A fragment of the IgG molecule related to Fab was discovered in the serum of partially hepatectomized rabbits by immunochemical analysis. Subsequent fractionation of the serum on carboxymethylcellulose, with the use of an immuno-sorbent, led to the isolation of this fragment in a purified form. Its sedimentation constant was found to be 5.2S. It thus corresponds to a bivalent IgG fragment. If partial hepatectomy was performed on animals receiving trasylol, a polyvalent proteinase inhibitor, before the operation and during the next 4 h, the presence of the Fab fragment could not be demonstrated. It is concluded that the Fab fragment appears as a result of hydrolysis of IgG by tissue proteases liberated from the liver cells damaged during operation.

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Authors
  1. D. N. Evnin
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  2. I. A. Tarkhanova
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  3. A. Ya. Kul'berg
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Evnin, D.N., Tarkhanova, I.A. & Kul'berg, A.Y. Isolation and immunochemical analysis of hydrolysis products of γG-globulin in the serum of partially hepatectomized rabbits. Bull Exp Biol Med 81, 386–389 (1976). https://doi.org/10.1007/BF00804927

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  • DOI: https://doi.org/10.1007/BF00804927

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