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Stability of the physicochemical parameters of DNA during prolonged keeping

  • Biochemistry and Biophysics
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

The physicochemical properties of purified high-polymer DNA isolated from calf thymus and sturgeon testis were investigated after its prolonged keeping (for 1.5–2 years) at 0–4°C in 1 M NaCl, as a precipitate under 70% ethanol, and in the freeze-dried state. DNA obtained from these sources can be kept for at least 1.5 years under the conditions tested without appreciable detriment to its degree of polymerization or its secondary structure. DNA from sturgeon testis, with similar initial physicochemical properties to those of thymus DNA, exhibits much greater stability when kept under the same conditions, indicating the strength of its molecular structure.

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Literature Cited

  1. S. A. Rogacheva, G. G. Rusinova, and É. G. Sharova, Tsitologiya,12, 912 (1970).

    Google Scholar 

  2. A. S. Spirin, in: Advances in Biological Chemistry [in Russian], Vol. 4, Moscow (1962), pp. 93–133.

  3. A. S. Spirin, Biokhimiya, No. 5, 656 (1958).

    Google Scholar 

  4. D. M. Spitkovskii, Biofizika, No. 4, 396 (1958).

    Google Scholar 

  5. E. E. Chebotarev, É. Z. Ryabova, and V. M. Indyk, Protective and Therapeutic Action of Exogenous DNA during Irradiation by Fast Neutrons [in Russian], Kiev (1974).

  6. E. Chargaff, in: The Nucleic Acids, Vol. 1, New York (1955), p. 526.

  7. E. R. Kay, N. S. Simmons, and A. L. Daunce, J. Am. Chem. Soc.,74, 1724 (1952).

    Google Scholar 

  8. O. H. Lowry, N. J. Rosebrough, and A. L. Farr, J. Biol. Chem.,193, 265 (1951).

    Google Scholar 

  9. B. Panjevac and J. Ristic, Bull. Inst. Nucl. Sci. (Belgrade),8, 159 (1958).

    Google Scholar 

  10. V. Pantic, N. Stosic, D. Kanazir, et al., Nature,194, 942 (1962).

    Google Scholar 

  11. G. Schmidt and S. Thannhauser, J. Biol. Chem.,161, 83 (1945).

    Google Scholar 

  12. L. Wicart and C. Venderely, C. R. Acad. Sci. (Paris),264, Ser. D, 857 (1967).

    Google Scholar 

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Authors
  1. A. V. Kolobov
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  2. Yu. P. Vainberg
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Kolobov, A.V., Vainberg, Y.P. Stability of the physicochemical parameters of DNA during prolonged keeping. Bull Exp Biol Med 81, 360–363 (1976). https://doi.org/10.1007/BF00804920

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  • DOI: https://doi.org/10.1007/BF00804920

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