Effect of products of destruction of tissue macrophages on hematopoietic stem cells
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Products of destruction of mouse peritoneal macrophages (MDP), obtained aseptically by freezing and thawing the cells three times, when injected intraperitoneally into syngeneic mice cause an increase in the number of splenic colony-forming units (CFUs) in the hematopoietic tissue of the bone marrow and spleen, revealed by Till and McCulloch's method. This increase is a true increase, for it was shown that the fraction of transplanted stem cells adsorbed by recipient's spleen in donor mice receiving MDP was relatively smaller than in the control. Besides an increase in the total number of splenic colonies a decrease was found in the number of erythroid colonies relative to the number of colonies of granulocytic and monocytic type. One possible mechanism of the effect of MDP on the number of CFUs may be modification of the hematopoiesis-inducing microenvironment, as is indicated by the increase in the number of colonies in mice into which normal hematopoietic tissue was transplanted after preliminary repeated injection of MDP. Other possible mechanisms of the observed effects also are examined, allowing for the fact that no direct effect of MDP on the stem cell could be found in experiments with preincubation of the bone marrow tissue with MDP before its injection into lethally irradiated mice.
Key Wordsproducts of destruction of macrophages hematopoietic stem cells
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