Abstract
Two-thirds of the liver was removed from August rats, and a suspension of azathioprene in 1% carboxymethylcellulose was injected in a dose of 40 mg/kg. The level of DNA synthesis was determined after 22, 48, 72 h with the aid of thymidine-H3, and the mitotic activity and the dimensions of the cells and their nuclei, were determined in the regenerating liver and intact spleen. Injection of azathioprene led to a decrease in the number of DNA-synthesizing cells in the regenerating liver, and spleen and depressed mitotic activity of the hepatocytes. Considerable development of hypertrophy of the cells and nuclei under the influence of azathioprene also were observed in the hepatocytes of the regenerating liver and the small lymphocytes of the white pulp of the spleen, i.e., the weight of the regenerating liver of the rats receiving azathioprene was restored by hypertrophy as well as mitotic division of the cells.
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Ryabinina, Z.A., Budik, V.M. & Lisnevskaya, E.A. Effect of azathioprene on DNA synthesis, mitotic activity, and hypertrophy of cells in the regenerating liver and intact spleen of rats. Bull Exp Biol Med 78, 1320–1322 (1974). https://doi.org/10.1007/BF00804372
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DOI: https://doi.org/10.1007/BF00804372