Abstract
In the early stage of Rauscher's leukemia, IgG capable of specific and selective fixation on the surface of the leukemic cells, as shown by the results of an indirect cytotoxic test and the indirect immunofluorescence test, can be isolated at low pH values from the spleen cells of C57BL/6 and BALB/c mice inoculated with Rauscher's virus together with Freund's complete adjuvant. The use of leukemic cells fixed with 1% glutaraldehyde as the immunosorbent makes it possible to isolate IgG, also possessing the properties of antibodies against antigens of the leukemic cell membrane, from the 7S-fraction of the serum.
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Ter-Grigorov, V.S., Dzagurov, S.G., Dorfman, N.A. et al. Isolation of 78-immunoglobulins (IgG) detectable on spleen cells and in mouse sera in the early stage of Rauscher's leukemia. Bull Exp Biol Med 78, 1302–1304 (1974). https://doi.org/10.1007/BF00804366
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DOI: https://doi.org/10.1007/BF00804366