Advertisement

Bulletin of Experimental Biology and Medicine

, Volume 79, Issue 3, pp 315–317 | Cite as

Cytochemical investigation of dynamics of RNA synthesis demonstrated in metaphase chromosomes of HeLa cells

  • A. A. Kucheryavenko
Experimental Biology
  • 17 Downloads

Abstract

The dynamics of synthesis of DNA and chromosomal RNA, demonstrated in metaphase chromosomes of HeLa cells, was investigated by methods of selective extraction of cells in mitosis from the monolayer and pulse labeling. The results showed that chromosomal RNA is itself synthesized chiefly at the end of the G1- and beginning of the S-period. The synthesis of chromosomal RNA was found in the G2-period or during mitosis.

Key Words

chromosomes RNA synthesis mitosis colchicine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    M. T. A. Martines and B. B. Fuks, Dokl. Akad. Nauk SSSR,187, 919 (1969).Google Scholar
  2. 2.
    B. B. Fuks, M. T. A. Martines, and A. A. Kucheryavenko, Vestn. Akad. Med. Nauk SSSR, No. 10, 35 (1971).Google Scholar
  3. 3.
    L. Djondjurov, Y. Markov, and R. Tsanev, Exp. Cell Res.,75, 442 (1972).Google Scholar
  4. 4.
    L. E. Feinendegen and V. P. Bond, Exp. Cell Res.,30, 393 (1963).Google Scholar
  5. 5.
    K. Habel and N. P. Salzman, in: Fundamental Techniques in Virology, New York (1969), p. 28.Google Scholar
  6. 6.
    D. S. Holmes, J. E. Mayfield, G. Sander, et al., Science,177, 72 (1972).Google Scholar
  7. 7.
    R. C. Huang and J. Bonner, Proc. Nat. Acad. Sci. (Washington),54, 960 (1965).Google Scholar
  8. 8.
    R. A. Jacobson and J. Bonner, Arch. Biochem.,140, 557 (1971).Google Scholar
  9. 9.
    J. D. Karkas, Proc. Nat. Acad. Sci. (Washington),69, 2288 (1972).Google Scholar
  10. 10.
    S. Penman, J. Molec. Biol.,17 117 (1966).Google Scholar
  11. 11.
    D. M. Prescott and M. A. Bender, Exp. Cell Res.,26, 260 (1962).Google Scholar
  12. 12.
    A. Rein and S. Penman, Biochim. Biophys. Acta,190, 1 (1969).Google Scholar
  13. 13.
    A. Sugino, S. Hirase, and R. Okazaki, Proc. Nat. Acad. Sci. (Washington),69, 1863 (1972).Google Scholar
  14. 14.
    G. Gerasima and L. J. Galmach, Exp. Cell Res.,30, 344 (1963).Google Scholar

Copyright information

© Plenum Publishing Corporation 1975

Authors and Affiliations

  • A. A. Kucheryavenko

There are no affiliations available

Personalised recommendations