Basic Research in Cardiology

, Volume 92, Issue 1, pp 1–7 | Cite as

Decrease in myocardial Na+-K+-ATPase activity and ouabain binding sites in hypercholesterolemic rabbits

  • W. -J. Chen
  • S. -Y. Lin-Shiau
  • H. -C. Huang
  • Y. -T. Lee
Original Contribution



The purpose of this study was to explore the effect of high dietary cholesterol on the lipid composition, Na+−K+-ATPase activity and ouabain receptor property of the myocardial sarcolemma.


Male New Zealand white rabbits were fed with standard chow or standard chow supplemented with 0.5% (w/w) cholesterol and 10% (w/w) coconut oil to induce hypercholesterolemia. After 8 weeks, the rabbits were sacrificed; a myocardial sarcolemma fraction was then prepared from the left ventricular myocardium and analyzed for lipid composition. Assay of Na+−K+-ATPase activity and3H-ouabain binding studies were performed in the myocardial sarcolemma from the control and cholesterol-fed rabbits.


The cholesterol content, but not the phospholipid content, of the sarcolemma was significantly greater in the cholesterol-fed group, thus, resulting in an increased cholesterol/phospholipid molar ratio in the cholesterol-fed group. In addition, a decrease in Na+−K+-ATPase activity was also found in this group. The decrease in Na+−K+-ATPase activity was selective, since the Mg++-ATPase and 5′-nucleotidase activities remained unchanged. In the3H-ouabain binding study, a decrease in the number of maximum binding sites, but not the binding affinity, for3H-ouabain was foundie the cholesterol-fed group.


High dietary cholesterol induces higher levels of cholesterol not only in the plasma, but also in the myocardial sarcolemma. These changes result in decreased myocardial Na+−K+-ATPase activity mediated by a reduction in the maximum number of binding sites for ouabain but not a change in binding affinity.

Key words

Hypercholesterolemia membrane lipid Na+−K+-ATPase ouabain receptor 


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Copyright information

© Steinkopff Verlag 1997

Authors and Affiliations

  • W. -J. Chen
    • 1
  • S. -Y. Lin-Shiau
    • 2
  • H. -C. Huang
    • 2
  • Y. -T. Lee
    • 1
  1. 1.Department of Internal Medicine College of MedicineNational Taiwan UniversityTaipeiTaiwan
  2. 2.Department of Pharmacology College of MedicineNational Taiwan UniversityTaipeiTaiwan

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