Bulletin of Experimental Biology and Medicine

, Volume 83, Issue 1, pp 71–74 | Cite as

Effect of cyclophosphamide on synthesis of antibodies and nonspecific immuno-globulins in mouse spleen cells

  • E. V. Sidorova
  • L. N. Fontalin
  • T. K. Novikova
  • L. A. Pevnitskii
Microbiology and Immunology


Intraperitoneal injection of cyclophosphamide (CP) into mice in a dose of 200 mg/kg body weight leads after 2–3 weeks to a marked acceleration of the formation of nonspecific immunoglobulins (NIG) by spleen cells in vitro. In the early period (3–7 days) after injection of CP the acceleration of NIG production was due mainly to synthesis of macroglobulins; after 2 weeks the formation of both macroglobulins and IgG was increased. Normal NIG synthesis was not restored until at least 1 month later. Injection of antigen (sheep's red cells) against the background of hyperproduction of NIG led to a decrease in the latter. The formation of antibodies against red cells in mice treated with CP was at a lower level at all times of the investigation than in control immunized animals.

Key words

cyclophosphamide antibodies nonspecific immunoglobulins 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    A. E. Gurvich, G. I. Drizlikh, and E. V. Sidorova, in: Immunochemical Analysis [in Russian], Moscow (1968), p. 234.Google Scholar
  2. 2.
    T. K. Novikova, L. A. Pevnitskii, and L. N. Fontalin, Byull. Éksp. Biol. Med., No. 5, 71 (1974).Google Scholar
  3. 3.
    L. N. Fontalin, L. A. Pevnitskii, V. V. Solov'ev, et al., Vestn. Akad. Med. Nauk SSSR, No. 7, 75 (1970).Google Scholar
  4. 4.
    M. A. Yumasheva, L. N. Fontalin, and A. M. Poverennyi, Byull. Éksp. Biol. Med., No. 5, 64 (1973).Google Scholar
  5. 5.
    J. Anderson, O. Sjöberg, and G. Möller, Eur. J. Immunol.,2, 349 (1972).Google Scholar
  6. 6.
    F. Miller and H. Metzger, J. Biol. Chem.,240, 3325 (1965).Google Scholar
  7. 7.
    G. D. Stockman, L. R. Heim, M. A. South, et al., J. Immunol.,110, 277 (1973).Google Scholar
  8. 8.
    P. Toivanen, A. Toivanen, and R. Good, J. Immunol.,109, 1058 (1972).Google Scholar
  9. 9.
    J. L. Turk, Proc. Roy. Soc. Med.,66, 805 (1973).Google Scholar

Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • E. V. Sidorova
  • L. N. Fontalin
  • T. K. Novikova
  • L. A. Pevnitskii

There are no affiliations available

Personalised recommendations