Abstract
With the aid of various activation systems, polycyclic aromatic hydrocarbons (PAH) were bound covalently with DNA. The following activation systems were used: with the microsomal fraction of rat liver, with I2, and with ascorbic acid and FeSO4. In all three systems the appearance of breaks due to the action of the activating systems was observed in the DNA. The plateau on the curve representing PAH binding in the system with microsomal fraction is explained not by a decrease in the rate of metabolism of the hydrocarbons to zero and not by the presence of a limited number of specific combining sites for PAH in DNA, but by equality between the velocities of two opposite processes; binding of the metabolites and their removal through degradation of DNA. Against the background of breaks in DNA produced by the activation systems it was impossible to find changes introduced into the sedimentation characteristics of DNA by covalently bound PAH.
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Karamysheva, A.F., Koblyakov, V.A. & Mironov, N.M. Characteristics of DNA during binding of polycyclic aromatic hydrocarbonsin vitro . Bull Exp Biol Med 87, 20–22 (1979). https://doi.org/10.1007/BF00802343
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DOI: https://doi.org/10.1007/BF00802343