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Adrenergic stimulation of rat hearts with severely reduced cytosolic adenine nucleotide pool and [ATP]/[ADP] ratio

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Summary

The effect of severe reduction of cytosolic adenine nucleotide (AdN) pool and [ATP]/[ADP] ratio (by 2-deoxyglucose treatment) on functional and metabolic responses of isovolumic rat heart to increased energy demand induced by coronary flow (CF) rise and isoproterenol (Iso) addition has been investigated. AdN-depleted hearts had reduced phosphocreatine (PCr, by 80%), ATP (by 75%), [ATP]/[ADP] (24 times) and pressure-rate product (PRP, by 60%). An elevation of CF was followed by the increase in PRP in control and AdN-depleted hearts by 40–45% with unchanged metabolic parameters. At increased CF, Iso caused a further rise in PRP in both groups due to elevation of heart rate; however maximal levels of PRP in the AdN-depleted group still remained lower than that of control (by 40%). Only in control experiments was Iso addition accompanied by an increase in the difference between left-vetricular end- and minimal diastolic pressure, cytosolic [Pi] and [ADP], and some decrease in PCr and [ATP]/[ADP]. These data imply that severely reduced cytosolic [ATP]/[ADP] does not prevent acceleration of Ca2+ turnover by Iso in cardiomyocytes, it but restricts maximal force development affecting the myofibrils.

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Authors and Affiliations

  1. Institute of Experimental Cardiology, Russian Cardiology Research Center, Academy of Medical Sciences, 3rd Cherepkovskaya str., 15A, 121552, Moscow, Russia

    V. V. Kupriyanov, O. V. Korchazhkina, V. L. Lakomkin & V. I. Kapelko

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  1. V. V. Kupriyanov
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  2. O. V. Korchazhkina
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  3. V. L. Lakomkin
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  4. V. I. Kapelko
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Kupriyanov, V.V., Korchazhkina, O.V., Lakomkin, V.L. et al. Adrenergic stimulation of rat hearts with severely reduced cytosolic adenine nucleotide pool and [ATP]/[ADP] ratio. Basic Res Cardiol 87, 139–147 (1992). https://doi.org/10.1007/BF00801961

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  • DOI: https://doi.org/10.1007/BF00801961

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