Abstract
A photometric (with the SPM scanning photometric microscope and the Wang-720C computer) investigation of DNA in lepra cells was carried out in series of paraffin sections stained by the Feulgen method. Considerable (up to 30–35%) fluctuations of the extinction values were found in individual sections in the same series. The aggregated results for the series were relatively uniform. It is concluded that to determine the DNA content reliably it is necessary to undertake photometric investigations on series of sections.
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Vishnevetskii, F.E. Cytophotometry of DNA in serial sections. Bull Exp Biol Med 86, 1675–1677 (1978). https://doi.org/10.1007/BF00801838
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DOI: https://doi.org/10.1007/BF00801838