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Presence of complementary RNA in chicken sarcoma cells and Rous sarcoma virus

  • Oncology
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

The subcellular localization of nucleotide sequences in Rous chicken sarcoma complementary to RNA of Rous sarcoma virus was investigated by RNA-RNA molecular hybridization. Samples of virion RNA labeled with radioactive iodine (125I) were annealed with RNA from different fractions (nuclei, mitochondria, free and membrane-bound polysomes) isolated from Rous chicken sarcoma cells. The formation of RNase-resistant hybrids between virus RNA-125I and RNA from mitochondria and membrane-bound polysomes was demonstrated; the relative frequency of occurrence of sequences complementary to virion RNA in the latter, moreover, was 446 times greater than in the former. The role of complementary ribonucleotide sequences is discussed.

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Authors
  1. Ya. D. Shaposhnikov
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  2. É. A. Ratovitskii
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  3. O. K. Kuznetsov
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Shaposhnikov, Y.D., Ratovitskii, É.A. & Kuznetsov, O.K. Presence of complementary RNA in chicken sarcoma cells and Rous sarcoma virus. Bull Exp Biol Med 85, 356–358 (1978). https://doi.org/10.1007/BF00801357

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  • DOI: https://doi.org/10.1007/BF00801357

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