Abstract
A method of microgel chromatography of proteins on a Sephadex G-75 (superfine) column with a working volume of 110–150 μl is described. By means of the method molecular weight fractionation of proteins can be carried out within the range 10−7–10−8 g in the course of 45–60 min.
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Literature Cited
D. G. Knorre and L. S. Sandakhchiev, in: Ultramicroanalysis of Nucleic Acids [in Russian], Moscow (1973), p. 7.
S. V. Kuz'min, in: Ultramicroanalysis of Nucleic Acids [in Russian], Moscow (1973), pp. 95–103.
H. Determann, Gel Chromatography [Russian translation from the German, Moscow (1970)] [English translation: Springer-Verlag, New York (1969)].
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Klement'ev, B.I. Molecular weight fractionation of proteins within the range 10−7–10−8 g by microgel chromatography. Bull Exp Biol Med 83, 756–757 (1977). https://doi.org/10.1007/BF00800773
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DOI: https://doi.org/10.1007/BF00800773