Abstract
Foci of heterotopic hematopoiesis were obtained by transplanting bone marrow of C57BL/6 or (CBAxC57BL)F1 mice beneath the kidney capsule of (CBA/T6T6xC57BL)F1 mice carrying a chromosomal translocation. Cytogenetic analysis of hematopoietic cells from these foci 20–120 days after transplantation showed that only the recipient's hematopoietic cells proliferate in 40% of the grafts, whereas the rest are mosaic and contain on average less than 20% of the donor's cells in both syngeneic and semisyngeneic systems. These characteristics remain stable for at least 4 months. It is concluded that the heterotopic focus is populated in one stage by a very small number (fewer than 10) hematopoietic stem cells. The stability of the clones is evidence that in the dynamic equilibrium of hematopoiesis the exchange of cells between different parts of the hematopoietic system is extremely small or is absent altogether.
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Udalov, G.A., Gurevich, O.A. & Chertkov, I.L. Origin of hematopoietic cells in syngeneic and semisyngeneic foci of heterotopic hematopoiesis. Bull Exp Biol Med 83, 693–695 (1977). https://doi.org/10.1007/BF00800752
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DOI: https://doi.org/10.1007/BF00800752