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Resistance of different forms of cytochrome P-450 of rat liver to factors destabilizing the microsomal membrane

  • Biochemistry and Biophysics
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

The effect of factors destabilizing the membrane of the liver microsomes on the spectral properties of cytochrome P-450 (P-448) was investigated in intact rats and rats receiving phenobarbital (PB) or 3-methylcholanthrene (MC). Considerable resistance of microsomes induced by PB and MC to enzymic and nonenzymic peroxidation of polyunsaturated fatty acids of membrane phospholipids was discovered. A clear difference was shown in the sensitivity of cytochrome P-448 and cytochrome P-450 of intact rats and rats receiving PB to in vitro treatment with sodium deoxycholate. The results indicate structural changes in the microsomal membrane during induction by PB and MC, which are two different types of inducers of the monooxygenases of the liver.

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Authors
  1. O. A. Gromova
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  2. N. E. Zakharova
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  3. V. V. Lyakhovich
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  4. I. B. Tsyrlov
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Gromova, O.A., Zakharova, N.E., Lyakhovich, V.V. et al. Resistance of different forms of cytochrome P-450 of rat liver to factors destabilizing the microsomal membrane. Bull Exp Biol Med 83, 653–656 (1977). https://doi.org/10.1007/BF00800740

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  • DOI: https://doi.org/10.1007/BF00800740

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