Abstract
A simple and reproducible method of monolayer cultivation of hepatocytes from 7–12-week human fetuses is suggested. Such cultures are capable of limited growth, they can be maintained without subculaute for 4–6 weeks, and they preserve certain specific characteristics: synthesis of such characteristic serum proteins as albumin and α-fetoprotein, the presence of a high glycogen content and high monoamine oxidase activity, and the characteristic reaction to high concentrations of glucocorticoid. These properties can be used in experimental genetics as markers or for selection purposes.
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Eraizer, T.L., Grinberg, K.N. & Vorsanova, S.G. Characteristics of human embryonic hepatocytes cultivated in vitro. Bull Exp Biol Med 79, 598–600 (1975). https://doi.org/10.1007/BF00800515
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DOI: https://doi.org/10.1007/BF00800515