Abstract
A simplified method of organ culture of human gonads is suggested. Altogether 190 gonads from 7–11-week embryos were grown on Millipore filters in penicillin flasks. During the first week of culture areas of central necrosis were observed in both the ovaries and the testes, but during subsequent cultivation these disappeared in some of the explants. Progressive development of germinative cells took place in the ovaries of 9–11-week embryos as far as the pachytene stage, whereas development in the ovaries of 7–9 week embryos did not reach the leptotene stage. Germinative cells during cultivation of the testes did not progress beyond the spermatogonium stage.
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Gapienko, E.F. Development of gonads of 7–11-week human fetuses in organ culture. Bull Exp Biol Med 79, 577–579 (1975). https://doi.org/10.1007/BF00800508
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DOI: https://doi.org/10.1007/BF00800508