Abstract
A method of determining activity of Na, K-ATPase in the fraction of unpurified synaptosomes isolated from weighed microsamples (2–3 mg wet weight of tissue) of rat cerebral cortex is described. The method is based on fluorometric measurement of ADP formed in the course of the ATPase reaction; it is highly sensitive and can be used to determine the Na, K-ATPase activity of membrane preparations with a protein content of 0.5–1.0 μg per sample.
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Samsonova, N.A., Rozhanets, V.V. Enzymic micromethod of determining membrane Na, K-ATPase activity in the rat cerebral cortex. Bull Exp Biol Med 86, 1413–1414 (1978). https://doi.org/10.1007/BF00800476
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DOI: https://doi.org/10.1007/BF00800476