Determination of “settling factor” (F) by cloning of hematopoietic stem cells in the mouse spleen and bone marrow
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The effects of distribution of injected colony-forming units (CFU) in the recipient were studied by the use of the “settling factor” (F) as the criterion. This factor was found from the number of colonies discovered in histological sections of the spleen and femoral bone marrow compared with the number of colonies distinguishable on the surface of the spleen. It was shown that the value of F both for the whole volume of the spleen and for the femoral marrow can be estimated by the method of macroscopic counting of colonies on the spleen. The value of F in this case was independent of the character of differentiation of the CFU. It was shown that the value of F can vary depending on the physiological state of the CFU population. After irradiation of bone marrow CFU in doses of 200, 400 and 600 R the value of F in the spleen was reduced, whereas in the bone marrow it was unchanged compared with the control.
Key Words“F” factor colony-forming units spleen bone marrow
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