Abstract
Aggregation of normal mouse fibroblast-like cells, placed on the surface of a Millipore filter, was investigated quantitatively. The addition of 10 μg/ml of cytochalasin B (a substance blocking active cell movements) or 0.1 μg/ml of colcemid (a substance disturbing polarization processes in cells and, consequently, interfering with directional cell movements) completely inhibits such aggregation. The inhibition is reversible—after removal of the agents the cells again aggregate. It is concluded that aggregation of cells in this experimental system requires active cell movement and cellular polarization.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
A. D. Bershadskii and V. I. Gel'shtein Ontogenez,4, 472 (1973).
A. D. Bershadskii and T. M. Lyustig, T sitologiya,17, 639 (1975).
Yu. M. Vasil'ev, I. M. Gel'fand, et al., T sitologiya,14, 80 (1972).
A. Ya. Fridenshtein, R. I. Rappoport, and E. A. Luria, Dokl. Akad. Nauk SSSR176, 452 (1967).
E. J. Ambrose and M. Ellison, Europ. J. Cancer,4, 459 (1968).
S. B. Carter, Nature,208, 1183 (1965).
S. B. Carter, Endeavour (Engl. ed.),31, No. 113, 77 (1972).
A. Harris, Exp. Cell. Res.,77, 285 (1973).
G. R. Martin and H. Rubin, Exp. Cell. Res.,85, 319 (1974).
M. S. Steinberg, J. Exp. Zool.,173, 395 (1970).
N. K. Wessells, J. F. Spooner, et al., Science,171, 135 (1971).
Rights and permissions
About this article
Cite this article
Bershadskii, A.D., Gel'shtein, V.I. Reversible inhibition of cellular aggregation by cytochalasin B and colcemid. Bull Exp Biol Med 81, 61–62 (1976). https://doi.org/10.1007/BF00800167
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00800167