Bulletin of Experimental Biology and Medicine

, Volume 85, Issue 2, pp 239–241 | Cite as

Immunomorphological study of the early stages of amyloidogenesis

  • L. N. Kapinus


The early stages of amyloidogenesis were studied in experiments on mice. Frozen sections through the spleen and other organs were incubated by the indirect Coons' method initially with pure rabbit antibodies against fibrillary protein of mouse amyloid, later with pure fluorescent goat antibodies against rabbit IgG. In the early stages, after one or two injections of casein, amyloid protein was observed to appear in the intercellular space and in the blood filling the lumen of the sinuses of the spleen and liver. The formation of typical deposits of amyloid in the spleen was complete by the 5th–7th day of the experiment after three to five injections of casein.

Key Words

amyloidogenesis amyloid antigen immunofluorescence 


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Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • L. N. Kapinus

There are no affiliations available

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