Bulletin of Experimental Biology and Medicine

, Volume 82, Issue 4, pp 1551–1554 | Cite as

Diurnal rhythms of cell proliferation in late precancerous changes induced in the liver by orthoaminoazotoluene

  • A. G. Mustafin


Orthoaminoazotoluene was administered to mice for 9 moths. Diurnal rhythms of mitotic activity and of the number of DNA-synthesizing cells were established by autoradiography with3H-thymidine in stages II (adenomatous nodules) and III (primary hepatomas) of carcinogenesis in the developing tumors and surrounding parenchyma of the liver. A monomodal rhythm of mitotic activity was demonstrated in the structures mentioned above, with the number of mitoses reaching a maximum at 4–7 a.m., whereas the diurnal rhythm of the index of labeled nuclei was bimodal, with maxima at 7 p.m. and 4 a.m. The mean diurnal values of both indices at stages II and III of hepatocarcinogenesis were considerably higher than in the surrounding noncancerous liver tissue.

Key Words

orthoaminoazotoluene late stages of hepatocarcinogenesis diurnal rhythms of mitosis and of DNA-synthesizing cells 


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Copyright information

© Plenum Publishing Corporation 1976

Authors and Affiliations

  • A. G. Mustafin

There are no affiliations available

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