A cytological analysis of the testicular tissue of the monkey grown in vitro as a trypsinized monolayer culture

  • L. B. Tsypkin
Experimental Biology


The investigations show that in the monolayer trypsinized culture of the monkey testicular tissue there is growth of epithelial and connective tissue elements. At first there is a prevalence of epithelial, and later — of connective tissue elements which, proliferating, actively depress the epithelial growth. Connective tissue cells undergo changes and form fibrous structures during the cultivation: they transform into flattened fibroblasts which are difficult to differentiate from epithelial cells. True (connective tissue) origin of these epithelium-like cells may be authentically established only with the aid of Heidenhain's strain, as well as by silver impregnation after Snesarev. These methods are recommended for cellular identification in monolayer cultures.


Public Health Epithelial Cell Connective Tissue Tissue Cell Monolayer Culture 
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Copyright information

© Consultants Bureau 1964

Authors and Affiliations

  • L. B. Tsypkin
    • 1
  1. 1.Institute of Poliomyelitis and Virus EncephalitisAMN SSSRMoscow

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