Abstract
L-cells arising from subcutaneous connective tissue and adipose tissue of C3H mice were cultured in nutrient medium with the concentration of bovine serum increased to 60%. Differentiation of some of the cells into fat cells took place, with the formation of structures similar to normal adipose tissue. The occurrence of differentiation was judged from the formation of characteristic signet-ring cells, whose cytoplasm was filled with neutral fat, giving a positive reaction for lipids when stained with a mixture of Sudan III and Sudan IV. It is concluded that the long existence of the cells in vitro and the intracellular changes accompanying the transformation process, including those of mutation character, do not render the cells incapable of differentiation.
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Literature Cited
V. G. Eliseev, M. Ya. Subbotin, Yu. I. Afanas'ev, et al., Fundamentals of Histology and Histological Techniques [in Russian], Moscow (1967).
A. A. Zavarzin and S. I. Shchelkunov, Textbook of Histology [in Russian], Leningrad (1954).
R. E. Kavetskii, Vopr. Onkol., No. 9, 3 (1975).
R. E. Kavetskii, V. I. Struk, V. A. Shuklinov, et al., in: Oncology [in Russian], No. 7, Kiev (1977), p. 73.
Yu. M. Olenov, Cellular Heredity, Cell Differentiation, and Carcinogenesis as Problems in Evolutionary Genetics [in Russian], Leningrad (1967).
B. S. Ruchkovskii, T. N. Oleinikova, V. A. Shuklinov, et al., in: Problems in Experimental Oncology [in Russian], No. 3, Kiev (1968), p. 30.
A. I. Serebrov, Vopr. Onkol., No. 8, 3 (1975).
N. S. Staroverova, Vopr. Onkol., No. 9, 3 (1961).
A. C. Braun, in: Cancer: A Comprehensive Treatise, Vol. 3, New York (1975), p. 3.
R. D. Cahn, in: The Stability of the Differentiated State, Berlin (1968), p. 58.
W. R. Earle, J. Nat. Cancer Inst.,3, 555 (1943).
W. R. Earle, E. J. Schilling, T. H. Stark, et al., J. Nat. Cancer Inst.,59, 715 (1977).
V. Spurna and M. Hill, Neoplasma (Bratislava),14, 11 (1967).
S. Vernecar, S. Gangal, and K. Ranadive, Ind. J. Exp. Biol.,8, 11 (1970).
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Department of General Mechanisms of Carcinogenesis, Institute for Problems in Oncology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 86, No. 8, pp. 242–245, August, 1978.
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Kavetskii, R.E., Shuklinov, V.A. Differentiation of a transformed culture of L-cells. Bull Exp Biol Med 86, 1105–1108 (1978). https://doi.org/10.1007/BF00799606
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DOI: https://doi.org/10.1007/BF00799606