Effect of human tonsillar lymphocyte extract on the macrophage migration inhibition test in guinea pigs exposed to various antigens

Abstract

Guinea pig peritoneal exudate cells were treated with human tonsillar lymphocyte extract in vitro or in vivo. The macrophage migration inhibition test was performed with normal cells and with cells treated with the extract. Old tuberculin, purified tuberculin, staphylococcal toxoid, and staphylococcal antiphagin were used as antigens. Lymphocyte extract without antigen did not affect macrophage mobility. Staphylococcal antiphagin without lymphocyte extract caused weak inhibition of cell migration. Preliminary treatment of the cells in vivo or in vitro with lymphocyte extract considerably increased the sensitivity of the cells to staphylococcal antiphagin. The remaining antigens did not inhibit macrophage migration whether before or after treatment of the cells with lymphocyte extract.