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Effect of hypothermia on metabolism in the liver during preservation

  • Biochemistry and Biophysics
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

The effect of hypothermia to different depths (18–20°C and 4–6°C) on carbohydrate metabolism and the degree of solubilization of the enzymes in the liver (lactate and glutamate dehydrogenases, urocaninase, deoxyribonuclease, glucose-6-phosphatase) during preservation of the unperfused organ was studied in 20 experiments on dogs. The effectiveness of preservation was assessed during subsequent normothermic perfusion for 2 h. After preservation of the liver at 18–20°C marked solubilization of the above-mentioned enzymes was observed, indicating a disturbance of the integrity of the cell membranes during preservation. After preservation of the liver at 4–6°C moderate utilization of the glycogen reserves in the liver and of sugar in the perfusion fluid was observed, suggesting uniform depression of metabolism in the liver and predominance of normal tissue respiration over glycolysis on restoration of the circulation in the organ.

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Authors
  1. E. A. Kvitsinskaya
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  2. D. B. Krivulis
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  3. Yu. A. Sorokin
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Kvitsinskaya, E.A., Krivulis, D.B. & Sorokin, Y.A. Effect of hypothermia on metabolism in the liver during preservation. Bull Exp Biol Med 86, 1033–1036 (1978). https://doi.org/10.1007/BF00799582

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  • DOI: https://doi.org/10.1007/BF00799582

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