Abstract
The effect of heparin on incorporation of thymidine-3H into A-1 cells in continuous culture was studied. Incubation of the cells in medium No. 199 containing heparin (1–200 units/ml) did not lead to any decrease in the percentage of labeled nuclei. The duration of the C2-and S-periods likewise was unchanged compared with the control. However, the intensity of incorporation of thymidine-3H (mean number of grains above one labeled nucleus) was significantly reduced. The results indicate that heparin affects the permeability of the plasmalemma but has no effect on DNA synthesis. The antimitotic action of heparin is probably connected with its effect on the cell surface.
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Blyumkin, V.N., Ul'yanova, L.I. Effect of heparin on incorporation of thymidine-3H into A-1 cells in continuous culture. Bull Exp Biol Med 82, 1402–1404 (1976). https://doi.org/10.1007/BF00799491
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DOI: https://doi.org/10.1007/BF00799491