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Effect of cocaine on tyrosine hydroxylase of rat hypothalamus

  • Pharmacology
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Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

The effect of cocaine (an adrenergic agent) on tyrosine hydroxylase of the rat hypothalamus was investigated in vivo (0.5 mg/kg) and in vitro (in concentrations of 10−6–10−5 M). Cocaine reduces Km for the coenzyme 6,7-dimethyl-5,6,7,8-tetrahydropterine (DMPH4) without altering the maximal reaction velocity. Internal administration of cocaine leads to a decrease in tyrosine hydroxylase activity after 10 min, especially of the membrane-bound form of that enzyme. After injection of cocaine a decrease in Km was found for DMPH4 and a decrease in the maximal reaction velocity. The decrease in the maximal reaction velocity is regarded as the result of the indirect action of cocaine.

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Literature Cited

  1. V. V. Zakusov, Byull. Éksp. Biol. Med., No. 11, 43 (1975).

    Google Scholar 

  2. M. F. Mineeva-Vyalykh, Vopr. Med. Khim., No. 2, 274 (1975).

    Google Scholar 

  3. V. A. Yakovlev, The Kinetics of Enzymic Catalysis [in Russian], Moscow (1965), p. 58.

  4. M. J. Besson, A. Cheramy, C. Gauchy, et al., Europ. J. Pharmacol.,22, 181 (1972).

    Google Scholar 

  5. R. T. Kuczenski and A. J. Mandell, J. Biol. Chem.,247, 3114 (1972).

    Google Scholar 

  6. M. Levitt, S. Spector, A. Sjoerdsma, et al.,: J. Pharmacol. Exp. Ther.,148, 1 (1965).

    Google Scholar 

  7. S. B. Ross and B. Brunfélter, J. Pharm. Pharmacol.,20, 283 (1968).

    Google Scholar 

  8. D. S. Segal, J. Y. Sullivan, R. Kuczenski, et al., Science,:173, 897 (1971).

    Google Scholar 

  9. S. Spector, A. Sjoerdsma and S. Udenfriend, J. Pharmacol. Exp. Ther.,147, 86 (1965).

    Google Scholar 

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Authors and Affiliations

  1. Academy of Medical Sciences of the USSR, USSR

    V. V. Zakusov (Academician)

Authors
  1. V. V. Zakusov
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  2. M. F. Mineeva-Vyalykh
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Additional information

Institute of Pharmacology, Academy of Medical Sciences of the USSR, Moscow. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol 82, No. 9, pp. 1071–1073, September, 1976.

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Zakusov, V.V., Mineeva-Vyalykh, M.F. Effect of cocaine on tyrosine hydroxylase of rat hypothalamus. Bull Exp Biol Med 82, 1340–1342 (1976). https://doi.org/10.1007/BF00799469

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  • DOI: https://doi.org/10.1007/BF00799469

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