Bulletin of Experimental Biology and Medicine

, Volume 83, Issue 2, pp 155–158 | Cite as

Investigation of the mechanism of action of ouabain and cyclic AMP of the transport of calcium ions by rat heart mitochondria

  • B. A. Badyshtev
  • S. B. Seredenin
Biochemistry and Biophysics
  • 20 Downloads

Abstract

The action of ouabain and cyclic AMP on the Ca-accumulating capacity and outflow of Ca2+ ions from loaded rat heart mitochondria was studied by the tetracycline probe method. In the course of the investigations no effect of ouabain on these processes was found. Cyclic AMP did not act on Ca binding by the mitochondrial membrane but it induced rapid liberation of Ca2+ from organelles loaded with these ions.

Key Words

ouabain cyclic AMP calcium mitochondria 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    L. G. Korkina, V. I. Sorokovoi, and Yu. A. Vladimirova, Stud. Biophys.,39, 177 (1973).Google Scholar
  2. 2.
    É. Sh. Matlina, Usp. Fiziol. Nauk, No. 4, 92 (1972).Google Scholar
  3. 3.
    A. B. Borle, J. Membrane Biol.,16, 221 (1974).Google Scholar
  4. 4.
    G. Brooker, Science,182, 933 (1973).Google Scholar
  5. 5.
    E. Carafoli, J. Molec. Cell. Cardiol.,7, 83 (1975).Google Scholar
  6. 6.
    A. H. Caswell and J. D. Hutchison, Biochem. Biophys. Res. Commun.,42, 43 (1971).Google Scholar
  7. 7.
    S. C. Harvey, Arch. Int. Pharmacodyn.,213, 222 (1975).Google Scholar
  8. 8.
    S. Katz and A. Tenenhouse, Br. J. Pharmacol.,48, 516 (1973).Google Scholar
  9. 9.
    K. S. Lee, S. A. Hong, and D. H. Kaug, J. Pharmacol. Exp. Ther.,172, 180 (1970).Google Scholar
  10. 10.
    A. Lehninger, in: Metabolism of the Myocardium (Proceedings of the First Soviet-American Symposium) [in Russian], Moscow (1975), pp. 178–194.Google Scholar
  11. 11.
    E. M. Tarjan and S. I. Kimata, Endocrinology,89, 385 (1971).Google Scholar

Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • B. A. Badyshtev
  • S. B. Seredenin

There are no affiliations available

Personalised recommendations