Bulletin of Experimental Biology and Medicine

, Volume 83, Issue 2, pp 129–132 | Cite as

Action of tetanus toxin and colchicine on synaptic membranes of the rat cerebral cortex

  • G. N. Kryzhanovskii
  • Yu. G. Sandalov
  • V. I. Rodina
  • V. V. Rozhanets
  • R. N. Glebov
Pathological Physiology and General Pathology
  • 11 Downloads

Abstract

Purified tetanus toxin (TT), in experiments in vitro, was shown to affect neither the Na,K-ATPase activity of the synaptic membrane fraction of the rat cerebral cortex nor the inhibition of Na,K-ATPase activity produced by electrical stimulation of a suspension of synaptic membranes, nor the binding of GABA-3H by synaptosomes. TT and colchicine (1 mM) reduced the osmotic sensitivity of the nerve endings. Colchicine, in low concentrations (10−5 to 10−3 M), does not affect Mg- and Na,K-ATPase but, in higher concentrations (10−2 M), it inhibits the activity of both ATPases considerably.

Key Words

synaptosomes electrical stimulation GABA binding colchicine osmotic sensitivity contractile proteins tetanus toxin 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    N. G. Bondarchuk, G. N. Kryzhanovskii, and A. Ya. Rozanov, Byull. Éksp. Biol. Med., No. 3, 39 (1973).Google Scholar
  2. 2.
    R. N. Glebov and G. N. Kryzhanovskii, Usp. Fiziol. Nauk, No. 4, 3 (1975).Google Scholar
  3. 3.
    R. N. Glebov and G. N. Kryzhanovskii, in: Problems in Brain Biochemistry [in Russian], Erevan (1976), p. 131.Google Scholar
  4. 4.
    R. N. Glebov, G. N. Kryzhanovskii, L. V. Malikova, et al., Biokhimiya,41, 975 (1976).Google Scholar
  5. 5.
    I. S. Gushchin, S. N. Kozhechkin, and Yu. S. Sverdlov, Byull. Éksp. Biol. Med., No. 8, 29 (1970).Google Scholar
  6. 6.
    G. N. Kryzhanovskii, Tetanus [in Russian], Moscow (1966).Google Scholar
  7. 7.
    G. N. Kryzhanovskii, R. N. Glebov, N. M. Dmitrieva, et al., Byull. Éksp. Biol. Med., No. 12, 24 (1974).Google Scholar
  8. 8.
    G. N. Kryzhanovskii, R. N. Glebov, N. M. Dmitrieva, et al., Byull. Éksp. Biol. Med., No. 1, 45 (1974).Google Scholar
  9. 9.
    G. N. Kryzhanovskii, R. N. Glebov, and A. A. Polgar, in: The Pathology of Membrane Permeability [in Russian], Moscow (1975), p. 82.Google Scholar
  10. 10.
    G. N. Kryzhanovskii, O. M. Pozdnyakov, et al., Byull. Éksp. Biol. Med., No. 12, 27 (1971).Google Scholar
  11. 11.
    G. N. Kryzhanovskii and O. P. Sakharova, Byull. Éksp. Biol. Med., No. 6, 36 (1972).Google Scholar
  12. 12.
    G. N. Kryzhanovskii, V. I. Fedorova, R. N. Glebov, et al., Byull. Éksp. Biol. Med., No. 4, 19 (1975).Google Scholar
  13. 13.
    N. I. Maisov, Yu. G. Sandalov, R. N. Glebov, et al., Byull. Éksp. Biol. Med., No. 1, 45 (1976).Google Scholar
  14. 14.
    Yu. G. Sandalov, R. N. Glebov, G. N. Kryzhanovskii, et al., Dokl. Akad. Nauk SSSR,224, 667 (1975).Google Scholar
  15. 15.
    V. V. Shevtsov, O. M. Pozdnyakov, I. I. Musin, et al., Byull. Éksp. Biol. Med., No. 1, 94 (1972).Google Scholar
  16. 16.
    S. Berl, S. Puszkin, and W. I. Nicklas, Science,179, 441 (1973).Google Scholar
  17. 17.
    D. R. Curtis and W. E. de Groat, Exp. Brain Res.,10, 208 (1968).Google Scholar
  18. 18.
    H. Feit and M. L. Shelanski, Biochem. Biophys. Res. Commun.,66, 920 (1975).Google Scholar
  19. 19.
    G. N. Kryzhanovskii (G. N. Kryzhanovsky), Arch. Pharmakol.,276, 247 (1973).Google Scholar
  20. 20.
    O. H. Lowry and J. A. Lopez, J. Biol. Chem.,162, 421 (1951).Google Scholar
  21. 21.
    I. Mellanby and V. P. Whittaker, J. Neurochem.,15, 205 (1968).Google Scholar
  22. 22.
    Y. Nomura and T. Segawa, J. Neurochem.,24, 1247 (1975).Google Scholar
  23. 23.
    W. E. Van Heyningen and P. M. Miller, J. Gen. Microbiol.,24, 107 (1961).Google Scholar

Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • G. N. Kryzhanovskii
    • 1
  • Yu. G. Sandalov
  • V. I. Rodina
  • V. V. Rozhanets
  • R. N. Glebov
  1. 1.Academy of Medical Sciences of the USSRUSSR

Personalised recommendations