Abstract
An enriched fraction of glial cells was isolated by centrifugation in a Ficollsucrose density gradient from bovine cerebral cortical tissue, and an actomyosinlike protein (AMLP) was obtained from it. The yield of the AMLP was 0.05% relative to glial cell protein. The AMLP was found to contain bound nucleotides and to give reversible association-dissociation reactions characteristic of AMLP under the influence of Mg2+ ions and ATP. ATPase of glial AMLP, which is activated by Ca2+ ions by a greater degree than Mg2+, differs from the AMLP of neuronal origin.
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Laboratory of General Pathology of the Nervous System, Institute of General Pathology and Pathological Physiology, Academy of Medical Sciences of the USSR, Moscow. Department of Biochemistry, Tbilisi University. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 83, No. 3, pp. 280–282, March, 1977.
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Sandalov, Y.G., Glebov, R.N., Kryzhanovskii, G.N. et al. Isolation and properties of an actomyosin-like protein from glial cells of bovine brain. Bull Exp Biol Med 83, 314–316 (1977). https://doi.org/10.1007/BF00799347
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DOI: https://doi.org/10.1007/BF00799347