Simultaneous determination of T, B, and mixed rosette-forming lymphocytes in human peripheral blood

  • S. Mueller
  • M. A. Stenina
Microbiology and Immunology


Preliminary treatment of erythrocytes in suspension with trypan blue did not affect their ability to take part in the spontaneous rosette formation test, but enabled them to be distinguished when mixed with other erythrocytes after staining with methyl green-pryronine in fixed preparations. The presence of a small subpopulation of lymphocytes binding both types of indicator particles was demonstrated by the use of a mixture of these, erythrocytes with erythrocytes sensitized by antibodies and complement.

Key words

rosette formation T and B lymphocytes 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    S. I. Donskov and E. A. Zotikov, Probl. Gematol., No. 7, 16 (1975).Google Scholar
  2. 2.
    N. G. Kochergin, “The functional state of the immunocompetent cells of patients with atopic dermatitis,” Candidate's Dissertation, Moscow (1975).Google Scholar
  3. 3.
    R. V. Petrov, M. A. Stenina, and K. A. Ledebev, Byull. Éksp. Biol. Med., No. 2, 197 (1976).Google Scholar
  4. 4.
    F. Aiuti and H. Wigzell, Clin. Exp. Immunol.,13, 171 (1973).Google Scholar
  5. 5.
    F. Aiuti, V. Lacava,. J. A. Garofalo, et al., Clin. Exp. Immunol.,14, 587 (1973).Google Scholar
  6. 6.
    A. Basten, N. L. Warner, and T. Mandel, J. Exp. Med.,135, 627 (1972).Google Scholar
  7. 7.
    J. W. Chiao, V. S. Pantic, and R. A. Good, Clin. Exp. Immunol.,18, 483 (1974).Google Scholar
  8. 8.
    J. W. Chiao, V. S. Pantic, and R. A. Good, Clin. Immunol. Immunopathol.,4, 545 (1975).Google Scholar
  9. 9.
    A. Dobozy, S. Uusz, J. Hunyadi, et al., Clin. Exp. Immunol.,23, 382 (1976).Google Scholar
  10. 10.
    S. S. Froland and J. B. Natvig, Transplant. Rev.,16, 114 (1973).Google Scholar
  11. 11.
    H. P. Lohrmann and L. Novikovs, Clin. Immunol. Immunol.,3, 99 (1974).Google Scholar
  12. 12.
    N. F. Mendes, S. S. Miki, and L. F. Peixinho, J. Immunol.,113, 531 (1974).Google Scholar
  13. 13.
    E. Shevach, R. Edelson, M. Frank, et al., Proc. Nat. Acad. Sci. USA,71, 863 (1974).Google Scholar
  14. 14.
    N. P. A. Silveira, N. F. Mendes, et al., J. Immunol.,108, 1456 (1972).Google Scholar
  15. 15.
    J. Wybran, M. C. Carr, and H. H. Fudenberg, J. Clin. Invest.,51, 2537 (1972).Google Scholar

Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • S. Mueller
  • M. A. Stenina

There are no affiliations available

Personalised recommendations