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Simultaneous determination of T, B, and mixed rosette-forming lymphocytes in human peripheral blood

  • S. Mueller
  • M. A. Stenina
Microbiology and Immunology
  • 10 Downloads

Abstract

Preliminary treatment of erythrocytes in suspension with trypan blue did not affect their ability to take part in the spontaneous rosette formation test, but enabled them to be distinguished when mixed with other erythrocytes after staining with methyl green-pryronine in fixed preparations. The presence of a small subpopulation of lymphocytes binding both types of indicator particles was demonstrated by the use of a mixture of these, erythrocytes with erythrocytes sensitized by antibodies and complement.

Key words

rosette formation T and B lymphocytes 

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Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • S. Mueller
  • M. A. Stenina

There are no affiliations available

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