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Bulletin of Experimental Biology and Medicine

, Volume 55, Issue 2, pp 160–162 | Cite as

A comparative immunoelectrophoretic study of the proteins of the placenta and of the fetal blood serum

  • D. Muzhnai
Biochemistry and Biophysics

Summary

By employing Levy-Polonovski and Wadsworth-Hanson immunoelectrophoretic methods with short trough, and also the Vasileisky method of stereoimmunological development it was shown that electrophoregrams of placental homogenate and umbilical cord blood serum possess identical components: 1) one in albumin fraction, 2) one in {ie162-1} fraction, 3) one in {ie162-2} fraction, and 4) one in γ-globulin fraction; cross-reacting components (one in {ie162-3} fraction and two in γ-globulin fraction), as well as related but not identical components in albumin fraction (spore). The placenta used in the experiments was duly washed of blood, so that, following immunoelectrophoretic tests by Grabar-Williams and antiserum development as against adult human serum in optimal concentration the arcs were barely perceptible. However, the same antiserum, applied to a human serum electrophoregram, gave eight quite distinct bands.

Keywords

Public Health Albumin Human Serum Cord Blood Blood Serum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Literature Cited

  1. 1.
    S. S. Vasileiskii, Biokhimiya, 5, 855 (1959).Google Scholar
  2. 2.
    P. Grabar and C. A. Williams, Biochim. biophys. Acta, 1953, v. 10, p. 193.Google Scholar
  3. 3.
    T. Itasaka, Okayama Igakkai Zasshi, 1958, v. 70, p. 2707.Google Scholar
  4. 4.
    H. Kusumoto, Ibid., 1957, v. 69, p. 2931.Google Scholar
  5. 5.
    G. Lévy and J. Polonovski, Bull. Soc. Chim. Biol. (Paris), 1958, v. 40, p. 1293.Google Scholar
  6. 6.
    Y. Miyoshi, Okayama Igakkai Zasshi, 1959, v. 71, p. 555.Google Scholar
  7. 7.
    O. Ouchterlony, Lancet, 1949, v. 256, p. 346.Google Scholar
  8. 8.
    D. Subrahmanyam and P. H. Maurer, J. Immunol., 1959, v. 83, p. 327.Google Scholar
  9. 9.
    M. Torimaru, Okayama Igakkai Zasshi, 1959, v. 69, p. 3073.Google Scholar
  10. 10.
    C. Wadsworth and L. A. Hanson, Int. Arch. Allergy, 1960, v. 17, p. 165.Google Scholar
  11. 11.
    E. Zapp, Mschr. Kinderheilk., 1960, Bd. 108, S. 120. As in original [Publisher's note].Google Scholar
  12. 12.
    E. Zapp, Mschr. Kinderheilk., 1960, Bd. 108, S. 120. As in original [Publisher's note].Google Scholar

Copyright information

© Consultants Bureau Enterprises, Inc. 1964

Authors and Affiliations

  • D. Muzhnai
    • 1
  1. 1.Laboratory of Immunochemistry, Research Institute of Obstetrics and GynecologyMinistry of Health of the RSFSRUSSR

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