Current Genetics

, Volume 10, Issue 3, pp 187–195 | Cite as

Saccharomyces cerevisiae strains sensitive to inorganic mercury

II. Effect of glucose
  • Etsuo Sakamoto
  • Hitomi Urata
  • Bun-ichiro Ono
Original Articles
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Summary

Saccharomyces cerevisiae strains sensitive to inorganic mercury (Ono and Sakamoto 1985) did not grow well on the medium rich in glucose and poor in peptone. This growth inhibition, like growth inhibition caused by inorganic mercury, was relieved by exogenous tyrosine. Sugars such as fructose and mannose were as inhibitory as glucose, but glycerol was not at all. Galactose was inhibitory but not so much as glucose. Agal2l mutation (defective in galactose uptake) partly relieved growth inhibition caused by excess galactose. Moreover, it was found that some of revertants which gained ability to grow well in the presence of excess glucose were defective in the glucose uptake. From these observations, we conclude that growth inhibition of the inorganic mercury sensitive strains by excess sugar is a consequence of the catabolite regulation. In other words, the inorganic mercury sensitive strains are hyper-sensitive to the catabolite regulation due to the presence of theHGS2-1 allele.

Key words

Saccharomyces cerevisiae Inorganic mercury Catabolite regulation Sugar uptake 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Etsuo Sakamoto
    • 1
  • Hitomi Urata
    • 1
  • Bun-ichiro Ono
    • 1
  1. 1.Laboratory of Environmental Hygiene Chemistry, Faculty of Pharmaceutical SciencesOkayama UniversityOkayamaJapan

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