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Quantitative Bestimmung primärer aliphatischer Alkohole (C6-C10) mit Hilfe einer pyridinnukleotidunabhängigen Alkoholdehydrogenase ausPseudomonas putida

Enzymatic determination of primary aliphatic alcohols (C6-C10) with an NAD(P)-independent alcohol dehydrogenase fromPseudomonas putida

  • Organische Chemie Und Biochemie
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Abstract

A new enzymatic method for microdetermination of longer chain alcohols has been established withNAD(P)-independent alcohol dehydrogenase from an alkane-oxidizing strain ofPseudomonas putida. The alcohols were estimated spectrophotometrically by measuring the formazan formation from the corresponding tetrazolium salt. Amounts of longer chain alcohols up to 3 nmol could be assayed by reading the endpoint of the reaction, even in presence of a 1000-fold exess of ethanol.

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Authors and Affiliations

  1. Bereich Biochemie der Sektion Biowissenschaften, Karl-Marx-Universität Leipzig, DDR-7010, Leipzig, Deutsche Demokratische Republik

    Sibylle Vorberg, Wulfdieter Schöpp & Hermann Tauchert

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  1. Sibylle Vorberg
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  2. Wulfdieter Schöpp
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  3. Hermann Tauchert
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Vorberg, S., Schöpp, W. & Tauchert, H. Quantitative Bestimmung primärer aliphatischer Alkohole (C6-C10) mit Hilfe einer pyridinnukleotidunabhängigen Alkoholdehydrogenase ausPseudomonas putida . Monatsh Chem 114, 563–569 (1983). https://doi.org/10.1007/BF00798611

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  • DOI: https://doi.org/10.1007/BF00798611

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