Abstract
Transformation ofBacillus subtilis was carried out intraperitoneally in mice. The frequency of transformation was considerably reduced by intraperitoneal injection of bovine deoxyribonuclease 1 (DNase 1) into the animals in a dose of 3–5 μg. Immune rabbit γ-globulins, containing antibodies against bovine DNase 1, inhibit the activity of DNase 1 in vivo, thus protecting the transforming DNA against the hydrolytic action of that enzyme. The model suggested can be used to search for ways of preserving a nucleic acid introduced into an animal for the purposes of genetic engineering.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
S. E. Bresler, V. L. Kalinina, and D. A. Perumov, Biokhimiya, No. 5, 907 (1963).
V. A. Drozhennikov, M. A. Guberniev, and A. A. Perelaznyi, in: Current Problems in Cellular Immunology and Immunogenetics [in Russian], Sofia (1973), p. 224.
M. B. Maksimovich, S. P. Lisovaya, and M. S. parfenova, Zh. Mikrobiol., No. 11, 58 (1974).
C. Paoletti, C. Gosse, and J. B. Le Pecq, Biokhimiya, No. 4, 647 (1963).
A. A. Pokrovskii, in: Chemical Bases of Processes of Vital Activity [in Russian], Moscow (1962), p. 311.
V. S. Shapot, Nucleases [in Russian], Moscow (1968), p. 72.
E. J. Cohn, L. E. Strong, W. L. Hughes, et al., J. Am. Chem. Soc.,68, 459 (1946).
E. Ephrati-Elisur, P. Srinivasan, and S. Zamenhof, Proc. Nat. Acad. Sci. USA,47, 56 (1961).
S. Fuchs, P. Cuatrecasas, D. A. Ontjes, et al., J. Biol. Chem.,244, 943 (1969).
E. A. Kabat and M. M. Mayer, Experimental Immunochemistry, Springfield (1958), p. 97.
M. Kunitz, J. Gen. Physiol.,33, 349 (1950).
M. McCarty, J. Exp. Med.,90, 543 (1949).
C. R. Merril and H. Stanbro, Z. Pflanzenphysiol.,72, 371 (1974).
J. F. Morrow, S. N. Cohen, A. C. Y. Chang, et al., Proc. Nat. Acad. Sci. USA,71, 1743 (1974).
J. Spizizen, Proc. Nat. Acad. Sci. USA,44, 1072 (1958).
Author information
Authors and Affiliations
Additional information
Research Laboratory of Experimental Immunobiology, Academy of Medical Sciences of the USSR, Moscow. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 84, No. 7, pp. 68–70, July, 1977.
Rights and permissions
About this article
Cite this article
Zhukov-Verezhnikov, N.N., Drozhennikov, V.A., Stolyarova, L.G. et al. Transformation ofBacillus subtilis in mice after immunological suppression of exogenous deoxyribonuclease 1 activity. Bull Exp Biol Med 84, 999–1001 (1977). https://doi.org/10.1007/BF00798529
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00798529