Concerning vesiculocytosis

Abstract

During the preparation of specimens from blood and tissues for detection of autoantibody-producing plaque-forming cells, if hypertonic (10–15%) salt solutions are used instead of physiological saline a phenomenon of false plaque formation takes place through the liberation of the cell contents into the surrounding medium and pycnosis of the cells. This phenomenon takes place with the blood karyocytes of animals of different species and of man when various salts are used, such as sodium nitrate, ammonium sulfate, calcium chloride, etc. False plaque formation in hypertonic solutions does not take place with all cells in the preparation, i.e., it depends on their state. The phenomenon is particularly strongly expressed in the period of maximal intensity of the cellular immune response.