Molecular Biology Reports

, Volume 8, Issue 1, pp 45–49 | Cite as

Involvement of histone H1 in the structure of the linker DNA in nucleosomes as revealed by nucleases

  • J. J. Lawrence
  • P. Goeltz
Article

Abstract

This report describes experiments designed to study the organization of the linker DNA in nucleosomes. When rat liver nucleosomes (145 to 188 base pairs in length) were digested by Exonuclease III and then by nuclease S1 a series of bands of sizes 90-102-112-125-135-142-154-166-172-181-bases was observed in denaturing electrophoretic gels. Digestion of H1-depleted nucleosomes under the same conditions results in a series of products of sizes (10.4) xn in base (n≤14) only. This result is interpreted as reflecting a particular arrangement of linker DNA under the influence of histone H1.

Keywords

Base Pair 

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Copyright information

© Dr W. Junk Publishers 1981

Authors and Affiliations

  • J. J. Lawrence
    • 1
  • P. Goeltz
    • 1
  1. 1.Laboratoire de Biologie Moléculaire et Cellulaire, Départment de Recherche FondamentaleCentre d'Etudes Nucléaires de GrenobleGrenoble cedexFrance

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