Basic Research in Cardiology

, Volume 88, Issue 2, pp 120–129 | Cite as

Tunnel capillaries in hypertrophied myocardium of rats with aorto-caval fistula

  • A. Ratajska
  • E. Fiejka
  • M. Maksymowicz
  • Z. Gawlik
Original Contributions
  • 22 Downloads

Summary

Male inbred rats (Wag) with aorto-caval fistula were used as a model of volume-overloaded heart hypertrophy. Hearts were taken after 1, 2, and 6 months postoperatively for immunohistochemical, histological and ultrastructural investigations. Immunohistochemical staining with some anti-extracellular matrix antibodies and ultrastructural findings allowed us to recognize intracardiocytic tunnels which were intracellular invaginations of plasmalemma surrounded by basement membrane. Inside the tunnels endothelial cells forming well-developed capillaries were entrapped. Tunnel capillary formation is discussed as an adaptive response to increased cardiac work due to volume overload.

Key words

Volume overload heart hypertrophy fibronectin laminin tunnel capillaries 

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References

  1. 1.
    Anversa P, Capasso JM, Ricci R, Sonnenblick EH, Olivetti G (1989) Morphometric analysis of coronary capillaries during physiologic myocardial growth and induced cardiac hypertrophy: A review. Int J Microcirc Clin Exp 8:353–363Google Scholar
  2. 2.
    Anversa P, Palackal T, Sonnenblick EH, Olivetti G, Capasso JM (1990) Hypertensive cardiomyopathy: myocyte nuclei hyperplasia in the mammalian heart. J Clin Invest 85:994–1003Google Scholar
  3. 3.
    Bishop SP, Dillon P, Naftilan J, Reynolds R (1980) Surface morphology of isolated cardiac myocytes from hypertrophied hearts of aging spontaneously hypertensive rats. Scanning Electr Micr 2:193–199Google Scholar
  4. 4.
    Dolber PC, Spach MS (1987) Picrosirius red staining of cardiac muscle following phosphomolybdic acid treatment. Stain Technol 62:23–26Google Scholar
  5. 5.
    Flaim SP, Minteer WJ, Nellis SH, Clark DP (1979) Chronic arteriovenous shunt: evaluation of a model for heart failure in rat. Am J Physiol 236:H698-H704Google Scholar
  6. 6.
    Gerdes AM, Cambell SE, Hilbelink DR (1988) Structural remodeling of cardiac myocytes in rats with arteriovenous fistulas. Lab Invest 59:857–861Google Scholar
  7. 7.
    Hansen-Smith FM, Watson L, Lu DY, Goldstein I (1988) Griffonia simplicifolia I: Fluorescent tracer for microcirculatory vessels in nonperfused thin muscles and sectioned muscle. Microvasc Res 36:199–215Google Scholar
  8. 8.
    Hatt PY, Rakusan K, Gastineau P, Laplace M (1979) Morphometry and ultrastructure of heart hypertrophy induced by chronic volume overload. (Aorto-caval fistula in the rat). J Mol Cell Cardiol 11:989–998Google Scholar
  9. 9.
    Hudlicka O, West D, Kumar S, El Khelly F, Wright AJA (1989) Can growth of capillaries in the heart and skeletal muscle be explained by the presence of an angiogenic factor? Br J Pathol 70:237–246Google Scholar
  10. 10.
    Hudlicka O, Wright AJA, Ziada AMAR (1986) Angiogenesis in the heart and skeletal muscle. Canadian J Cardiol 2:120–123Google Scholar
  11. 11.
    Ingber DE, Madri JA, Folkman J (1987) Endothelial growth factors and extracellular matrix regulate DNA synthesis through modulation of cell and nuclear expansion. In Vitro 23:387–394Google Scholar
  12. 12.
    Imamura K (1978) Ultrastructural aspect of left ventricular hypertrophy in spontaneously hypertensive rats: a qualitative and quantitative study. Japanese Circ J 42:979–1002Google Scholar
  13. 13.
    Kawamura K, Kashii C, Imamura K (1976) Ultrastructural changes in hypertrophied myocardium of spontaneously hypertensive rats. Japanese Circ J 40:1119–1145Google Scholar
  14. 14.
    Kawamura K, Tohda K, Kobayashi M, Masuda H, Shozawa T (1990) Fine structure of capillary proliferation in myocardium of volume overloaded rats. In: Piiper J (ed) Oxygen Transport to Tissue. XII, Plenum Press, New York, pp 387–394Google Scholar
  15. 15.
    Krogh A (1919) The number and distribution of capillaries in muscle with calculations of the oxygen pressure head necessary for supplying the tissue. J Physiol 52:409–415Google Scholar
  16. 16.
    Linzbach AJ (1960) Heart failure from the point of view of quantitative anatomy. Am J Cardiol 5:370–382Google Scholar
  17. 17.
    Michel JB, Salzmann JL, Ossondo Nlom M, Bruneval P, Barres D, Camilleri JP (1986) Morphometric analysis of collagen network and plasma perfused capillary bed in the myocardium of rats during evolution of cardiac hypertrophy. Basic Res Cardiol 81:142–154Google Scholar
  18. 18.
    Rakusan K, Moravec J, Hatt PY (1980) Regional capillary supply in normal and hypertrophied rat heart. Microvasc Res 20:319–332Google Scholar
  19. 19.
    Rakusan K, Wicker P, Samad MA, Healy B, Turek Z (1987) Failure of swimming exercise to improve capillarization in cardiac hypertrophy of renal hypertensive rats. Circ Res 61:641–647Google Scholar
  20. 20.
    Ratajska A, Gawlik Z, Fiejka E (1991) Some extracellular matrix elements as markers of capillary tunnels in hypertrophied rat heart. Experientia 47:965–968Google Scholar
  21. 21.
    Reitsma W (1973) Formation of new capillaries in hypertrophic skeletal muscle. Angiology 24:45–57Google Scholar
  22. 22.
    Sawada K, Kawamura K (1991) Architecture of myocardial cells in human cardiac ventricles with concentric and eccentric hypertrophy as demonstrated by quantitative scanning electron microscopy. Heart & Vessels 6:129–142Google Scholar
  23. 23.
    Schweigerer LG, Neufeld G, Friedman J, Abraham JA, Fiddes JC, Gospodarowicz D (1987) Capillary endothelial cells express basic fibroblast growth factor, a mitogen that promotes their own growth. Nature 325:257–259Google Scholar
  24. 24.
    Shekhonin BV, Guriev SB, Irgashev SB, Koteliansky VE (1990) Immunofluorescent identification of fibronectin and fibrinogen/fibrin in experimental myocardial infarction. J Mol Cell Cardiol 22:533–541Google Scholar
  25. 25.
    Shekhonin BV, Domogatsky SP, Idelson GL, Koteliansky VE (1988) Participance of fibronectin and various collagen types in the formation of fibrous extracellular matrix in cardiosclerosis. J Mol Cell Cardiol 20:501–508Google Scholar
  26. 26.
    Sulaiman AR, Borg DS (1989) Vascularizes muscle fibers: etiopathogenesis and clinical significance. J Neurol Sci 92:37–54Google Scholar
  27. 27.
    Terracio L, Borg TK (1988) Factors affecting cardiac cell shape. Heart Failure 4:114–124Google Scholar
  28. 28.
    Timpl R, Rohde H, Gehron-Robey P, Rennard SI, Toidart JM, Martin GR (1979) Laminin — a glycoprotein from basement membranes. J Biol Chem 254:9933–9937Google Scholar
  29. 29.
    Tomanek RJ, Palmer PJ, Peffer GL, Schreiber KL, Eastham CL, Marcus ML (1986) Morphometry of canine arteries, arterioles and capillaries during hypertension and left ventricular hypertrophy. Circ Res 58:38–46Google Scholar
  30. 30.
    Tomanek RJ, Wessel TJ, Harrison DG (1991) Capillary growth and geometry during long-term hypertension and myocardial hypertrophy in dogs. Am J Physiol 261:H1011-H1018Google Scholar
  31. 31.
    Weber KT, Janicki JS, Pick R, Capasso J, Anversa P (1990) Myocardial fibrosis and pathologic hypertrophy in the rat with renovascular hypertension. Am J Cardiol 65:1G-7GGoogle Scholar
  32. 32.
    Wolf R, Goebel HH, Gutmann L, Schochet S (1991) Capillaries within human skeletal muscle fibers. Path Res Pract 187:857–863Google Scholar
  33. 33.
    Wohlfart G (1951) Dystrophia myotonica congenita. Histopathologic studies with special reference to changes in the muscles. J Neuropath Exp Neurol 10:109–124Google Scholar

Copyright information

© Steinkopff-Verlag 1993

Authors and Affiliations

  • A. Ratajska
    • 1
  • E. Fiejka
    • 1
  • M. Maksymowicz
    • 2
  • Z. Gawlik
    • 1
  1. 1.Department of Pathological AnatomyMedical Academy of WarsawWarsawPoland
  2. 2.Maria Sklodowska-Curie Memorial Institute of OncologyWarsawPoland

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