Abstract
The effect of unfractionated preparations of RNA and histones isolated from intact and regenerating lymphoid tissue on the survival and growth of leukemic lymphocytes in syngeneic recipients was studied. The inhibitory action of histones on leukemic growth was well-marked but was independent of the morphological and functional state of the tissue at the time of their extraction from it. Electrophoresis in polyacrylamide gel showed no appreciable differences in the fractional composition of histones from intact and regenerating tissues. Preliminary treatment of the leukemic cells with RNA from regenerating tissue reduced the survival rate and increased the life span of the mice compared with animals receiving cells incubated with RNA from intact tissue. A spectrophotometric study of the Melting profile of the RNA preparations revealed differences in the ratio between the thermostable and unstable components of RNA from intact and regenerating tissues.
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Svirnovskii, A.I., Levin, V.I. & Nikolaichik, V.V. Comparative antileukemic action of RNA and histones from intact and regenerating homologous tissue. Bull Exp Biol Med 78, 1055–1058 (1974). https://doi.org/10.1007/BF00796666
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DOI: https://doi.org/10.1007/BF00796666