Abstract
The effect of stimulation of immunoglobulin synthesis by combined cultivation of lymph gland cells of immune mice and intact bone marrow cells, separated by membrane filters, was studied with the use of radioisotpes and immunosorbents. An increase in immunoglobulin synthesis was observed in the case of separation of the cell populations by Millipore membranes with a pore size of 25 nm and of 1.0–0.3 μ. No stimulation effect was observed if the cells were separated by cellophane membranes. Intensification of immunoglobulin synthesis was evidently the result of a humoral factor secreted by the cells of one of the adjacent populations.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Literature Cited
A. E. Gurvich, G. I. Drizlikh, and E. V. Sidorova, in: Immunochemical Analysis [in Russian], Moscow (1968), p. 234.
A. A. Mikhailova, R. V. Petrov, and L. A. Zakharova, Dokl. Akad. Nauk SSSR, No. 4, 209 (1971).
A. A. Mikhailova, R. V. Petrov, and L. A. Zakharova Dokl. Akad. Nauk SSSR,203, No. 4, 943 (1972).
R. E. Click, Z. Benck, and B. J. Alter, Cell. Immunol.,3, 264 (1972).
M. Feldmann and A. Basten, J. Exp. Med.,136, 49 (1972).
R. M. Gorezynski, R. G. Miller, and R. A. Phillips, J. Immunol.,108 547 (1972).
R. V. Petrov and A. A. Mikhailova, Cell. Immunol.,5 3 (1972).
A. Schimpl and E. Wecker, Nature,237, 13 (1972).
Rights and permissions
About this article
Cite this article
Zakharova, L.A., Galkina, N.S. Interaction in vitro between cells of immune lymph glands and intact bone marrow separated by a millipore membrane. Bull Exp Biol Med 78, 1034–1036 (1974). https://doi.org/10.1007/BF00796660
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00796660