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The effect of desoxyribonucleic acid on the radiosensitivity ofα-chymotrypsin in an artificial DNA-α-chymotrypsin complex

  • Biochemistry and Biophysics
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Bulletin of Experimental Biology and Medicine Aims and scope

Summary

The effect of DNA on the radiosensitivity of a protein included into the nucleoprotein complex was studied. Since biological activity, the change of which may conveniently serve as a criterion of protein disintegration, cannot yet be detected in the protein constituents of natural nucleoproteins, the authors used an artificial DNA-α-chymotrypsim complex for this investigation. It was found that after x-ray irradiation (10.000 and 50.000 r) of solutions of these complexes and of free α-chymotrypsim (control) the free enzyme is much more resistant to the action of the radiation than the same enzyme combined with DNA. The formation of different complexes may possibly be one of the factors which intensifies or reduces the radiosensitivity of the individual biological components of the cell.

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Literature Cited

  1. B.S. Diskina, Biokhimiya21, 482, (1956). Original Russian pagination. See C. B. translation.

    Google Scholar 

  2. B.S. Diskina and D.M. Spitkovskii, Biofizika,3, 633 (1958).

    Google Scholar 

  3. A.M. Kuzin, Radiobiology [in Russian] (Moscow, 1957).

  4. J.H. Northrop, et al., Crystalline Enzymes [Russian translation] (Moscow, 1950).

  5. A.S. Spirin, Biokhimiya23, 656 (1958). Original Russian pagination. See C. B. translation.

    Google Scholar 

  6. D.M. Spitkovskii Biofizika,3, 396 (1958).

    Google Scholar 

  7. D.M. Spitkovskii, V.S. Tongur, and B.S. Diskina, Biofizika,3, 129 (1958).

    Google Scholar 

  8. V.S. Tongur, N.P. Golubeva, et al., Biofizika2, 469 (1957).

    Google Scholar 

  9. D. Mazia and J. Blumenthal, J. Cell. Comp. Physiol.35, 171(1950).

    Google Scholar 

  10. A.E. Mirsky and A.W. Pollister, J. Gen. Physiol.,30, 117(1946).

    Google Scholar 

  11. M.G. Sewag, D.B. Lackman, and J. Smolens, J. Biol. Chem.124, 425 (1938).

    Google Scholar 

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Authors and Affiliations

  1. Institute of Experimental Biology of the AMN SSSR, Moscow

    T. Yu. Ugarova & P. I. Tseitlin

Authors
  1. T. Yu. Ugarova
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  2. P. I. Tseitlin
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Ugarova, T.Y., Tseitlin, P.I. The effect of desoxyribonucleic acid on the radiosensitivity ofα-chymotrypsin in an artificial DNA-α-chymotrypsin complex. Bull Exp Biol Med 50, 697–699 (1961). https://doi.org/10.1007/BF00796039

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  • DOI: https://doi.org/10.1007/BF00796039

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