Bulletin of Experimental Biology and Medicine

, Volume 46, Issue 6, pp 1526–1527 | Cite as

The argento- and mercurimetric determination of the content of SH- groups in the serum and brain homogenate of the cat

  • Iu. M. Torchinskii
Methods
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Summary

A simultaneous titration of SH-groups by HgCl2 in phosphate buffer was undertaken for the determination of the question whether a noticable oxidation (“disappearance”) of SH-groups takes place in the blood serum and in the brain homogenate under the effect of ammonium ion in their titration by AgNO3 in diluted ammoniac buffer. Experiments demonstrated that the results of the argento- and mercurimetric determination of the concentration of SH- groups in the blood serum and brain homogenate coincide completely. These data permit to conclude that in titrating the SH-groups by AgNO3 in diluted ammoniac buffer there is no appreciable oxidation or their “disappearance” under the effect of ammonium ion.

Keywords

Public Health Phosphate Ammonium Phosphate Buffer AgNO3 

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Copyright information

© Consultants Bureau, Inc. 1959

Authors and Affiliations

  • Iu. M. Torchinskii
    • 1
  1. 1.Laboratory of Histochemistry of the Institute of the BrainAMN SSSRMoscow

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