Bulletin of Experimental Biology and Medicine

, Volume 49, Issue 3, pp 312–313 | Cite as

A purified enzyme preparation obtained from Soviet pancreatin and its use for tissue “Trypsinization”

  • I. V. Vorontsov
  • O. S. Gudima
  • N. A. Kolesnikova
Methods
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Summary

The authors obtained an enzyme preparation — a mixture of tryspin, chymotrypspin and other pancreatic enzymes from the extract of Soviet pancreatin by subfecting it to double purification through activated charcoal. The activity of the resulting preparation approaches that of the crystalline tryspin and chymotryspin (750–1800 Chow Units/m and 30–50 mg% protein nitrogen). The enzyme preparation may be stored in solution in ampules for a period of 6 months at a temperature of +4°C without any noticeable loss of its effectiveness. The preparation successfully replaces trypsin-Difco in tissue cultivation. Its dosage was determined not by the weight of the dry pancreatin, but by the activity expressed by the proteolytic “Chow” Units, which enabled the authors to obtain reproducible results.

Keywords

Public Health Nitrogen Tissue Cultivation Charcoal Enzyme Preparation 

Literature Cited

  1. [1]
    L. V. Kolesnikov and N. E. Gorev, Voprosy Virusol. 1, 56 (1958).Google Scholar
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    L. N. Khristov, G. S. Bezverkhii, and I. E. Shulyapin, Voprosy Virusol. 3, 56 (1956).Google Scholar
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    B. Chow and M. Peticolas, J. Gen. Physiol.32, 17 (1948).Google Scholar
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    R. Dulbecco and M. Vogt, J. Exper. Med.99, 167 (1954).Google Scholar
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    J. S. Youngner, Proc. Soc. Exper. Biol. Med.85 202 (1954).Google Scholar

Copyright information

© Consultants Bureau Enterprises, Inc. 1960

Authors and Affiliations

  • I. V. Vorontsov
    • 1
  • O. S. Gudima
    • 1
  • N. A. Kolesnikova
    • 1
  1. 1.(Moscow)

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