Abstract
Fractionation of suspensions of rat bone marrow or spleen cells yielded fractions in which activity of DNA-dependent DNA-polymerase and aspartate-carbamoyl transferase and incorporation of14C-thymidine were two or three times greater than in the initial suspension. Depending on the osmotic concentration of the original 35% solution of bovine serum albumin, the cells synthesizing DNA most actively were concentrated in fractions Nos. 5–6 (370 milliosmoles) or Nos. 2–3 (380 milliosmoles).
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Moskaleva, E.Y., Mazurik, V.K., Evseeva, N.K. et al. Isolation of cells most actively synthesizing DNA from hematopoietic organs by fractionation in an albumin density gradient. Bull Exp Biol Med 82, 1673–1676 (1976). https://doi.org/10.1007/BF00790382
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DOI: https://doi.org/10.1007/BF00790382