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Bulletin of Experimental Biology and Medicine

, Volume 80, Issue 2, pp 889–891 | Cite as

Acute cell death induced by inhibition of DNA synthesis in various parts of the gastrointestinal tract

  • O. S. Frankfurt
Pathological Physiology and General Pathology

Abstract

Injection of hydroxyurea into mice led after 4 h to degeneration of nearly all DNA-synthesizing cells in the crypts of the small intestine, but only about 10% of these cells in the epithelium of the glandular part of the stomach underwent necrosis under these conditions. Hydroxyurea inhibited DNA synthesis equally in both tissues. The resistance of the gastric cells, as shown by the criterion of acute death, reflected their ability to with-stand inhibition of DNA synthesis without degeneration. This property is perhaps preserved in tumors of the stomach and may be one factor determining their resistance to chemotherapy.

Key Words

stomach intestine DNA synthesis hydroxyurea cell death 

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Literature Cited

  1. 1.
    L. F. Larionov, The Chemotheraphy of Malignant Tumors [in Russian], Meditsina, Moscow (1962).Google Scholar
  2. 2.
    O. F. Frankfurt, “Proliferation and differentiation of cells in the stratified squamous epithelium of the mouse forestomach (autoradiographic investigation),” Tsitologiya, No. 2, 175 (1967).Google Scholar
  3. 3.
    E. Farber and R. Baserga, “Differential effects of hydroxyurea on survival of proliferating cells in vivo,” Cancer Res.,29, 136 (1969).PubMedGoogle Scholar
  4. 4.
    L. Lenaz, S. S. Sternberg, and F. S. Philips, “Cytotoxic effect of 1-βD-arabinofuranosyl-5-fluorocytosine and 1-β-D-arabinofuranosylcytosine in proliferating tissues in mice,” Cancer Res.,29, 1790 (1969).PubMedGoogle Scholar
  5. 5.
    M. W. Lieberman, R. S. Verbin, M. Landay, et al., “A probable role for protein synthesis in intestinal epithelial cell damage induced in vivo by cytosine arabinoside, nitrogen mustard, or X-irradiation,” Cancer Res.,30, 942 (1970).PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1976

Authors and Affiliations

  • O. S. Frankfurt
    • 1
  1. 1.Sector of Kinetics of Chemical and Biological Processes, Institute of Chemical PhysicsAcademy of Sciences of the USSRMoscow

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