Molecular Biology Reports

, Volume 16, Issue 1, pp 39–47 | Cite as

Structure and site of expression of a murine type II hair keratin

  • Edda Tobiasch
  • Jürgen Schweizer
  • Hermelita Winter
Articles

Abstract

We present here a 1770 bp-long cDNA which encodes a murine type II keratin. Sequence comparisons of the keratin with those of various type II keratins expressed in mouse epidermis and internal stratified epithelia reveal that the new keratin is unrelated to epithelial keratins. Rather the structural organization of its amino- and carboxyterminal domains and the high content of cysteine and proline residues in these regions suggest that the keratin represents a murine type II hair keratin. This assumption was confirmed by in situ hybridization which localized the mRNA of the keratin in upper cells of the hair cortex and in suprabasal cells of the central core unit of filiform papillae of the tongue. Hybrid selection analyses revealed that the keratin has a molecular weight of 58 kD. It remains to be seen whether the keratin corresponds to MHb 3 or MHb 4.

Key words

mouse epithelia keratin expression in situ hybridization hair keratin 

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Edda Tobiasch
    • 1
  • Jürgen Schweizer
    • 1
  • Hermelita Winter
    • 1
  1. 1.Institute of BiochemistryGerman Cancer Research CenterHeidelbergGermany

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