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Impaired endothelium-dependent vasodilation of coronary resistance vessels in severely stunned porcine myocardium

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Abstract

The effect of stunning on endothelium-dependent responses of resistance vessels in vivo remains of interest. We utilized the coronary pressure-flow relationship during maximal vasodilation in anesthetized swine to identify subtle changes in flow reserve within stunned myocardium. Prior to and following stunning, the coronary pressure-flow relationship during maximal doses of intracoronary adenosine was compared with that of the endothelium-dependent vasodilator ATP. In 11 anesthetized swine, 30 min of partial LAD occlusion and 40 min of reperfusion reduced fractional shortening from 16±4% to 6±5% (p<0.05). This caused a rigtward shift of the coronary pressure-flow relationships during infusions of either adenosine or ATP, suggestive of increased extra vascular compressive forces. With adenosine, the slope of the linear portion of the relationship (i.e., coronary pressures >30 mmHg) was 1.31±0.54 ml/min-mmHg at baseline and 1.30±0.55 ml/min-mmHg following stunning (NS). With ATP however, the slope decreased from 1.34±0.48 ml/min-mmHg at baseline to 1.08±0.47 ml/min-mmHg following reperfusion (p<0.05), indicating an attenuation of endothelium-dependent vasodilator capacity. In five of the animals, the slope of the pressure-flow relationship during intracoronary nitroprusside was unchanged post-stunning, side was unchanged post-stunning, which is similar to the adenosine results. In conclusion, the data support the hypothesis that endo-thelium-dependent vasodilation of resistance vessels in the intact animal is altered within severely stunned myocardium. The rightward shift of the coronary pressure-flow relation ships with both classes of vasodilators suggest that extra vascular factors may also play a role in limiting coronary flow reserve.

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Authors and Affiliations

  1. Division of Cardiology, University of Minnesota-VA Medical Center, 1 Veterans Drive, 55417, Minneapolis, Minnespota, USA

    E. O. McFalls, H. Ward & P. Fashingbauer

  2. Laboratory for Experimental Cardiology Thoraxcenter, Erasmus University, Rotterdam, The Netherlands

    D. J. Duncker

Authors
  1. E. O. McFalls
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  2. D. J. Duncker
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  3. H. Ward
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  4. P. Fashingbauer
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Supported by grants from the Minnesota Medical Foundation, American Heart Association-Minnesota Affiliate and VA Merit Review (E.O.M.)

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McFalls, E.O., Duncker, D.J., Ward, H. et al. Impaired endothelium-dependent vasodilation of coronary resistance vessels in severely stunned porcine myocardium. Basic Res Cardiol 90, 498–506 (1995). https://doi.org/10.1007/BF00788543

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  • DOI: https://doi.org/10.1007/BF00788543

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