A study of the properdin system
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This work deals with an experimental analysis of the two possible methods of properdin determination and their disadvantages. The authors suggest a new simple method which consists of the substitution of Rp by a guinea pig complement with the utilization of normal rat serum (characterized by high and stable properdin titer) as a standard. The optimal dilution of the complement, which loses its hemolytic activity after treatment with rat serum in 1:600–1:800 dilution in presence of zymosan, is determined in a preliminary experiment. The amount of the serum under investigation capable of giving the same result with the titrated dose of complement, is determined in the main experiment.
KeywordsPublic Health Experimental Analysis Hemolytic Activity Main Experiment Titrate Dose
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