Summary
This work deals with an experimental analysis of the two possible methods of properdin determination and their disadvantages. The authors suggest a new simple method which consists of the substitution of Rp by a guinea pig complement with the utilization of normal rat serum (characterized by high and stable properdin titer) as a standard. The optimal dilution of the complement, which loses its hemolytic activity after treatment with rat serum in 1:600–1:800 dilution in presence of zymosan, is determined in a preliminary experiment. The amount of the serum under investigation capable of giving the same result with the titrated dose of complement, is determined in the main experiment.
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Rozental, K.M., Savelvolf, G.B. A study of the properdin system. Bull Exp Biol Med 48, 1435–1439 (1959). https://doi.org/10.1007/BF00788366
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DOI: https://doi.org/10.1007/BF00788366