Bulletin of Experimental Biology and Medicine

, Volume 48, Issue 5, pp 1435–1439 | Cite as

A study of the properdin system

Communication I. A simplified method of estimation of properdin
  • K. M. Rozental
  • G. B. Savelvolf
Methods
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Summary

This work deals with an experimental analysis of the two possible methods of properdin determination and their disadvantages. The authors suggest a new simple method which consists of the substitution of Rp by a guinea pig complement with the utilization of normal rat serum (characterized by high and stable properdin titer) as a standard. The optimal dilution of the complement, which loses its hemolytic activity after treatment with rat serum in 1:600–1:800 dilution in presence of zymosan, is determined in a preliminary experiment. The amount of the serum under investigation capable of giving the same result with the titrated dose of complement, is determined in the main experiment.

Keywords

Public Health Experimental Analysis Hemolytic Activity Main Experiment Titrate Dose 

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Literature Cited

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Copyright information

© Consultants Bureau Enterprises, Inc. 1960

Authors and Affiliations

  • K. M. Rozental
    • 1
  • G. B. Savelvolf
    • 1
  1. 1.Division of Microbiology of the Institute of Experimental Medicine of the AMN SSSRLeningrad

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